ISOBMInternational Society for Oncodevelopmental Biology and Medicine
ISOBMInternational Society of Oncology and Biomarkers
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Phage isolated by panning with the six PSA MAbs were tested for binding to 39 MAbs from the ISOBM workshop for characterization of antibodies against PSA by phage IFMA.
To study whether the epitopes found were recognized by other MAbs, we studied the reactivity of 19 peptides with a panel of MAbs from the ISOBM workshop.
In addition to these official bodies, several professional organizations have projects concerning immunoassay standardization, e.g., IFCC, the International Union for Pure and Applied Chemistry (IUPAC), ISOBM, the College of American Pathologists (CAP), NCCLS, and the American and Canadian societies for clinical chemistry, AACC and CFCC.
Specificity and affinity of 26 monoclonal antibodies against the CA 125 antigen: first report from the ISOBM TD-1 workshop.
Summary report on the ISOBM TD-4 Workshop: analysis of 56 monoclonal antibodies against the MUC1 mucin, San Diego, CA, November 17-23, 1996.
Epitope specificity of 30 monoclonal antibodies against cytokeratin antigens: the ISOBM TD5-1 Workshop.
Immunohistochemical characterization of 22 monoclonal antibodies against the CA125 antigen: 2nd report from the ISOBM TD-1 Workshop.
A pairing study was performed to determine whether the 15 antibodies could be paired with a set of reference anti-PSA antibodies included in the International Society for Oncodevelopmental Biology and Medicine (ISOBM) workshop (see below).
The ability of each of the 15 antibodies to inhibit binding of the 12 reference ISOBM workshop antibodies was also determined.
Pairing studies were performed to compare the epitope specificities of the antibodies produced with that of each other, with commercially available antibodies, and binding inhibition with ISOBM workshop antibodies.
A recent epitope-mapping workshop conducted by the International Society of Oncodevelopmental Biology and Medicine (ISOBM) has defined the binding characteristics of monoclonal antibodies reactive with CA 125 and has identified two major regions on the antigen, which are called OC125-like and M 11-like epitopes (5).