ISOLAB

AcronymDefinition
ISOLABInternational Symposium on Origins of Life and Astrobiology
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The specimens underwent erosive demineralization by immersion in 1.0% citric acid (pH 3.6, 10 mL/specimen) for 90 seconds, four times a day for five days under constant agitation (70 rpm) (Shaker, Isolab Laborgerate GmbH, Wertheim, Germany) [18, 26, 27].
Supernatant (5.5 [degrees]L/well) was loaded onto precast, agarose isoelectric focusing (IEF) gels (Isolab, Akron, OH).
and Isolab, both members of the Acrolab Group of companies.
To confirm this observation, we compared the Hb [A.sub.2] values obtained by HPLC and anion-exchange microchromatography (Quik-Sep; Isolab) in nine samples from Hb D heterozygotes.
Subsamples were sent to the laboratory Isolab (Netherlands) for carbon and hydrogen stable isotope analyses.
Supernatant (5.5 [micro]L/well) was loaded onto precast, agarose isoelectric focusing (IEF) gels (Isolab, Akron, OH).
For the past 9 years, this laboratory has used isoelectric focusing (IEF; Isolab) to screen neonates for hemoglobin disorders.
Microcolumn anion-exchange chromatography (MAEC; Quik-Sep[TM] Hemoglobin [A.sub.2] Test System; Isolab) was used as the comparison method for Hb [A.sub.2] only.
In the cord-blood study Hb types, including Hb Bart's and Hb Constant Spring (Hb CS), were also confirmed by isoelectric focusing (Isolab).
Recent development of quantitative determination of phenylalanine with the Wallac Quantase phenylalanine screening assay kit [2,3] and the Isolab phenylalanine test kit has provided other methods for newborn PKU screening.
Erythrocyte lysates were analyzed by starch gel- or cellulose acetate electrophoresis [1] or by isoelectrofocusing [2] with commercial agar plates (Isolab).