strips were cut into half and measured in the gamma counter to determine the percentage of labelled peptide (origin) to free radionuclide (solvent front).
99mTc-clarithromycin was completely stable in human serum during incubation as determined by ITLC
. It was found that 97% labeling was found at 24 hours of incubation with very little increase in free 99mTcO4 and reduced or hydrolyzed 99mTc at 37oC.
The [sup.68]Ga percentage of unbound and bound [sup.68]Ga-DOTA-TBIA101 was analyzed by ITLC
as described earlier.
Free [sup.111]In in the radiolabeled DOTA-J591 preparations was determined using the ITLC
method with a silica gel-impregnated glass fiber support and a mobile phase of 1% DTPA (pH 5.5).
Quality controls were performed using both instant thin layer chromatography (iTLC
) and reversed phase-HPLC (RP-HPLC).
Monitoring of all complexes was made by paper chromatography (PC) (Agilent, USA), instant thin layer chromatography (ITLC
) (Agilent, USA) and RP-HPLC (Shimadzu, Japan).
This final report describes the activities and outcomes of the Interactive Technology Literacy Curriculum (ITLC
Our in vitro stability results may not reflect real condition, because iTLC
might not be able to identify the disassociated[[sup.131]I] that might also be absorbed by protein(s) in blood.
The radiochemical purity of the radiolabeled complex was determined by Paper Chromatography (PC) and Instant Thin Layer Chromatography (ITLC
Radionuclide incorporation yield and radiochemical purity of radiolabeled DANBIRT were characterized by instantaneous thin layer chromatography (ITLC
) and high-performance liquid chromatography (HPLC), respectively, following previously published methods .