ITNFIn the Near Future
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TNF is a major proinflammatory cytokine induced by BCG infection and can be observed intracellularly (iTNF), on the cell surface as transmembrane (tmTNF) and in a soluble TNF form (sTNF) [22, 29-32].
To determine if low doses of BCG were enough to activate TNF and sTNFRs, we measured iTNF and tmTNF by flow cytometry and sTNF, sTNFR1, and sTNFR2 levels by ELISA.
* ITNF: theft of data by a current or former employee with low or no probability of fraudulent intent.