ITRAQIsobaric Tags for Relative and Absolute Quantitation
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Two-dimensional gel electrophoresis (2-DE), two dimensional difference-in-gel-electrophoresis (2D-DIGE), stable isotope labeling with amino acids in cell culture (SILAC), and isobaric tags for relative and absolute quantification (iTRAQ) are some of the commonly used quantitative proteomics tools.
In the present study, both the Western blot result and the iTRAQ result showed that the expression of Mapt in the hippocampus was reduced in depressive status but was increased significantly after EA pretreatment.
Total proteins of each group were analyzed by iTRAQ (AB Sciex, Framingham, Massachusetts, USA), liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS).
Through the application of iTRAQ technology, we obtained 458,154 total spectra for the two biological replicates using a mass spectrometer TripleTOF 5600 (Table 3).
A recent case-control study based on the LC-MS/MS and iTRAQ found 50 altered proteins (39 overexpressed and 11 subexpressed); three of them were validated through ELISA: alpha-enolase, IL-18, and KLK13 [127].
Noirel et al., "iTRAQ underestimation in simple and complex mixtures: the good, the bad and the ugly," Journal of Proteome Research, vol.
Plasma biomarkers of early AR were evaluated by iTRAQ (isobaric tags for relative and absolute quantification)-MALDI-TOF/TOF by comparison of meticulously selected patients with biopsy-proven AR and stable controls (9).
Na tecnica de iTRAQ, tambem e utilizada a marcacao de proteinas com etiquetas e identificacao por MS.
To explore the possible mechanisms, we used isobaric tags for relative and absolute quantification (iTRAQ) techniques combined with multidimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to perform a quantitative proteomic analysis.
Yu et al., "iTRAQ proteomics analysis reveals that PI3K is highly associated with bupivacaine-induced neurotoxicity pathways," Proteomics, vol.