Moshfe, "A consistent PCR-RFLP assay based on ITS-2
ribosomal DNA for differentiation of Fasciola species," Iranian Journal of Basic Medical Sciences, vol.
Caption: Figure 1: Agarose gel electrophoresis of PCR product of ITS-2
gene of L.
Within the regions of moderate variability, the genes for p-tubulin, TRAP (C1, C2 and C4) or the intergenic regions ITS-1 and ITS-2
have been used.
incognita (Eisenback et al., 1981), and amplification of rDNA and and partial sequence (ITS-I and ITS-2
) showed sequence homology with that of M.
Similitud de las secuencias amplificadas de las regiones ITS1-5.8S e ITS-2
del ADN ribosomal con las depositadas en el GeneBank de los aislamientos de Colletotrichum spp., obtenidos de papaya Pococi recolectada en el canton de Guacimo.
All the isolates generated a single prominent band on agarose gel which included partial sequence of 18S gene, complete sequence of ITS-1, 5.8S gene, ITS-2
and partial sequence of 28S gene.
Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, as inferred from ITS-2
Ribosomal DNA is present in all organisms and is composed of several regions (genes and spacers) that evolve at different rates (Hillis and Dixon, 1991); (5) The internal transcribed spacer regions (ITS-1 and ITS-2
) have been used at species and intraspecific levels of many taxa for distinguishing the different forms (Carbone and Kohn, 1993; Bowles and McManus, 1993; Hsiao et al., 1994; Buckler IV et al., 1997).
gattii specific primers that target the gene encoding the rDNA internal transcribed regions 1 (ITS-1) and 2 (ITS-2
) were used in a nested PCR reaction as described by Rappelli et al.
rDNA sequencing of Gnathostoma species (nematoda) and elucidation of the species causing human gnathostomiasis in the Americas.
canis en muestras de suelo, mediante amplificacion del transcrito interno (ITS-2
) del ADN ribosomal (ADNr) del parasito.
"Andean" and "non-Andean" ITS-2
and mtCytB haplotypes of Triatoma infestans are observed in the Gran Chaco (Bolivia): population genetics and the origin of reinfestation.