Disposable supplies such as Petri dishes, acrodisc filters, centrifuge tubes for media storage and pipette tips used during IVEP make the IVEP work seem expensive but the use of disposable materials is more beneficial and convenient in terms of eliminating contamination and ensuring purity and safety of the prepared media and the produced embryos.
In order to improve on IVEP results, this study ensured the expiry date of all reagents and media were noted and cross-checked at all times prior to use.
Prolonging the period of ovary collection may significantly affect the viability of the oocytes for IVEP.
The method was also used within the IVEP system reported here.
Not all oocytes should be used for IVEP because they lack the potential to undergo normal maturation and will eventually end up as degenerated COCs after in-vitro fertilization.
To ensure a successful fertilization of the oocytes during IVEP, the sperm cells that are used must be viable, motile, capacitated and capable of expressing acrosome reaction.
In vitro embryo culture, like other procedures of IVEP is carried out inside a water-jacketed incubator with 5% C[O.
Back-up equipment must be available, to avoid compromising the IVEP activity.
However, in order to produce optimal results in IVEP for the Boran breed, further studies may be necessary to shed more light on influence of nutrients and BCS of the cow on its embryo production in vitro.
By contrast, hormonal treatment steps are eliminated whenever embryos are produced in an IVEP setup, making this method of obtaining embryos a better option.
Utilization of the IVEP technology in Kenya can be used to carry out value addition to indigenous cows.
When IVEP and OPU technologies are combined, OPU enables a cow to provide over 100 ova per ovary per month for sale.