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The present RT-PCR assay was found specific for FMDV, as it was unable to detect IBDV and PPRV viruses.
It was the hypothesis of this study that the BGM-70 adapted and propagated IBDV could be well adapted and propagated on a microcarrier using bioreactor system.
The antiviral activity against IBDV and ARV was also evaluated by determining the viral titer (TCID50) in cultures of chicken fibroblasts.
The wild type IBD virus was reported to be extremely difficult to adapt to cell culture especially the very virulent IBDV (vvIBDV) unless passaged few times in chicken embryonated eggs (CEE) .
The chicks were divided as into four experimental groups: group A (control-only drinking water), group B (Aloe vera treated only), group C (vaccinated with IBDV vaccine), and group D (IBDV vaccinated and Aloe vera treated).
Chickens infected with IBDV between 3 and 6 weeks of age develop clinical IBD which may result in death but those infected at less than 3 weeks of age usually have few or no clinical signs.
Desde hace mas de treinta anos se han utilizado las vacunas vivas atenuadas para el control del IBDV (89, 98, 98).
This phenomenon can occur among different serotypes of the same virus, for example IBDV with intermediate and pathogenic strains  and it can also occur between different viruses, as between infectious bronchitis virus (IBV) and Newcastle disease virus (NDV)  or avian pneumovirus .Therefore, the major purpose of this investigation is to study the immunosuppressive effects of IBD vaccines schedule being used in Pakistan on the ND vaccine.
Infectious Bursal Disease Virus (IBDV) belongs to the family of Birnaviridae, which consists of naked viruses characterized by a bi-segmented, double strand RNA genome and is the causative agent of a highly infectious disease affecting young chickens (Gumbro disease) (1).
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