infantum antibodies by using an immunofluorescence antibody test (7); we tested all 408 dogs and 5 cats for the parasites' DNA by using real-time PCR (8).
Overall, we obtained positive results for 16.7% (95% CI 11.2%-22%) of dogs by immunofluorescence antibody test and 3.1% (95% CI 1.5%-4.8%) of dogs by PCR (Table), indicating that a large proportion of the canine population from Bosnia and Herzegovina has been exposed to or is infected by L.
For serologic diagnosis, an immunofluorescence antibody test
kit for A.
Screening for SAFV-specific antibodies was conducted by using an indirect immunofluorescence antibody test
on SAFV-infected Vero cells as described (9).
was detected by the immunofluorescence antibody test
All samples with inhibition [greater than or equal to] 20% were subsequently tested in an immunofluorescence antibody test
(IFAT) (7) with appropriate species-specific fluorescein isothiocynate conjugates.
by indirect immunofluorescence antibody test
, with cultured promastigotes of L.
A definitive diagnosis requires detection of the virus in the lesion by electron microscopy, viral culture, histopathological examination of the biopsy material, immunofluorescence antibody tests
or other serological examinations.4
Indirect immunofluorescence antibody tests
were negative for St.