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ITS1Internal Transcribed Spacer 1
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Results of analysis of methylation level in six CpG sites (26S ribosomal RNA gene, 26S-18S ribosomal RNA intergenic spacer, 18S ribosomal RNA gene, internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2) are presented in Table 4.
Leishmania typing: The ribosomal internal transcribed spacer 1 (ITS1) was subjected to PCR, using the LITSR and L.5.8S primers (25).
BRIUMSc internal transcribed spacer 1, partial sequence; 5.8S ribosomal###1160###1160###99%###0.0###99%
delavayi Franch.) based on the sequences of nrDNA internal transcribed spacer 1 (ITS1) regions.
The basic local alignment search tool ( analysis of partial nucleotide sequence of the internal transcribed spacer 1 and 5.8S ribosomal RNA gene sequence showed 98% homology to already reported Candida sp.
infantum was confirmed by PCR amplification of the internal transcribed spacer 1 region of the parasite (13), followed by restriction fragment length polymorphism analysis of the internal transcribed spacer 1 region amplicon (14).
Consensus distance-based tree generated from the infecting amastigote's internal transcribed spacer 1 sequence and homologous sequences from other related human Leishmaniainfected samples.
After extracting DNA from tissue samples by using the Quick-DNA Universal kit (Zymo Research, Irvine, California, USA), we performed PCR and sequencing of the ribosomal internal transcribed spacer 1 (6) to achieve typing of Leishmania spp.
By using 3 DNA sequences commonly used in the molecular systematics of filarial parasites (the nuclear internal transcribed spacer 1 [ITS1]-based ribosomal DNA sequence [7] and the mitochondrial 12S and cytochrome c oxidase subunit 1 genes [6]), we confirmed M.
Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.
yongonense strains isolated from patients in Italy; these strains were identified by sequencing the 16S rRNA, hsp65, rpoB, and sodA genes and the internal transcribed spacer 1 (ITS1) region.
yongonense in the internal transcribed spacer 1 region and in a 1,384-bp region of the hsp65 gene and found 2 mismatches in a 420-bp fragment of the sodA gene (99.5% similarity).
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