, noting no non-refuted indication of normal JCV
integration in the literature.
Established in January 1994, the Japan Committee, Vaccines for the World's Children (JCV
) is dedicated to eradicating throughout Asia the six main vaccine-preventable diseases.
Untreated urine (5 [micro]L; diluted 1:10 in distilled water) was examined with a quantitative TagMan PCR assay that detects BKV as well as JCV
. Each 50-[micro]L PCR assay contained 5 [micro]L of template, 500 nM each of the primers PVTM for and Pvback, 100 nM PVProbe exonuclease probe (for sequence data see Table 1), 100 [micro]M each of the deoxynucleotide triphosphates (Gibco), 5 [micro]L of 10x amplification buffer, 1 [micro]M ROX, and 2 U of Platinum Taq DNA polymerase (Gibco).
is found to infect more than 70% of the adult human population as a productively persistent infection, PML is a rare disease.
Levels of BKV and JCV
in blood and urine were detected using real-time quantitative polymerase chain reaction (PCR).
Of these, 20 samples from 17 patients initially tested positive for JCV
; further testing at the Centers for Disease Control and Prevention in Atlanta confirmed the findings in 7 specimens from 6 patients, said Ms.
In addition, eight novel HPyVs have been discovered, at least one of which (MCV) does not appear to follow the traditional disease course described above for JCV
and BKV .
A test to identify JCV
antibody status marks a significant step forward in the personalization of clinical management of MS patients who may benefit from Tysabri.
is the first blood test to receive FDA marketing authorisation for the detection of antibodies to the polyomavirus JC virus (JCV
) for stratifying risk for progressive multifocal leukoencephalopathy (PML).
After antigen retrieval with ethylenediaminetetraacetic acid at pH 8 and microwaving at 900 W (2 steps of 3 minutes, followed by 2 steps of 4 minutes), immunoperoxidase staining was performed using a polyclonal antibody against the VP1 through VP3 proteins of simian virus 40 (dilution, 1:20000; Lee Biomolecular Research Labs, San Diego, Calif), which cross-reacts with human BKV and JCV
. (24) The reactions were detected by means of the streptavidin-biotin method and were revealed using diaminobenzidine as the chromogen.
(8) conducted a study for the detection of the human polyomavirus JC (JCV
), using PCR.