In order to evaluate the effect of JJN in treating MG patients more accurately, we compared the miRNAs expressions of normal people, MG patients (pretreatment), and MG patients (treatment), respectively.
It was of statistical significance to use Wilcoxon signed rank test to assess the difference before and after JJN treatment.
On the heat maps, we noted that there were big changes in the miRNAs expression profiles of MG (pretreatment)/normal, MG (treatment)/normal, and MG (before and after treatment), which indicated that effects of TCM compound preparations JJN in treating MG patients may be realized via regulation of miRNA expression profilles.
This showed the differences of different samples of MG patients before and after treatment using JJN. Among them, hsa-let-7b-5p had the highest degree in the network.
According to our previous studies, JJN, a formula designed according to the TCM theories and clinical experience, has been used to treat MGI (I and II type) patients in China for decades.
Among them, the miRNA expression change in MG group before and after treatment may have occurred due to therapeutic effects of Chinese herbal compound JJN on MG patients.
Based on the abovementioned microarray bioinformatics analysis, we obtained that Chinese herbal compound JJN plays its therapeutic effect in treating MG patients by regulating differences in miRNA expression profiles, differences in the certain functions of miRNA expression profiles, changes in miRNA expression pathways, and other ways.