In this study, we presents the association of BFT traits with the GRIP1 gene using an F2 intercross population between KNP and Landrace pigs.
Pools of genomic DNA samples from the parental lines (KNP [n = 5] and Landrace [n = 5]) of the F2 intercross were re-sequenced to detect SNP markers across the whole genome, including the GRIP1 gene (ENSSSCG00000000478), using the HiSeq2000 massively parallel sequencing technology (Illumina Inc., San Diego, CA, USA).
Following the genome-wide linkage analysis to identify QTLs for BFT traits, a positional candidate gene analysis was conducted using an intercross between KNP and Landrace pigs.
Duroc pigs had the locus that corresponded to a high PERV insertional rate of 76.9%, followed by Landrace pigs with a rate of 59.1%, KNPs with a rate of 57.9%, Yorkshire pigs with a rate of 50.0% and Berkshire pigs with a rate of 17.4%.
On the other hand, the two selected PERV-C loci from NIH miniature pig BAC library indicated that each had the same integrated rates of 73.7% in KNPs. In NIH miniature pigs, the integration rate (40%) of PERVs at the C2-6C locus was relatively lower than that (90%) of the C4-2G locus.
The aim of this study was to investigate PERV insertional variations in different pig breeds using 11 selected PERV loci from Korean native pig (KNP) and National Institutes of Health (NIH) miniature pig bacterial artificial chromosome (BAC) libraries.
The Korean native pig (KNP) has a black coat color and a capacity to produce tender meat which is high in muscular fat, but low in cholesterol; and has more unsaturated fat than saturated fat.
Therefore, we determined a high-density linkage map of all porcine autosomes by using genotypes of the Illumina Porcine SNP60K Bead chip, and used line-cross model analysis to identify the QTL that may determine meat quality difference between KNP and YS.
We selected SNP markers that satisfied the following two conditions: i) the SNP marker allele frequency difference between the [F.sub.0] KNP (n = 16) and the Yorkshire (n = 8) population is >0.700; and ii) the PIC value of the individual SNP marker in the [F.sub.2] (n = 252) population is >0.300.
(2007) performed genome scans to detect Mendelian and parent-of-origin QTL for growth and body composition traits in a cross population of KNP and Landrace.
A resource population consisting of three generations was generated by crossing five KNP sires and ten Landrace dams at the National Livestock Research Institute (NLRI), Songhwan, Korea.
The full (partial) model included the probabilities of inheriting KNP and Landrace alleles at a testing position, while the paternal or maternal models included only probabilities of paternal or maternal inheritance.