KSFM


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AcronymDefinition
KSFMKeratinocyte Serum-Free Medium (liver medication)
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After 2 weeks of co-culture, floating sphere cells were dissociated with 0.25% trypsin-EDTA, filtered with a 40-[micro]m strainer to obtain the single cell suspension, resuspended in Matrigel (BD Biosciences, Bedford, MA) with KSFM (1:1, vol:vol), plated in the 24-well plate, and incubated at 37[degrees]C overnight to solidify before adding 1 mL KSFM.
The next day, 1 mL of KSFM growth medium was added on top of the solidified Matrigel mixture.
Swings:+ 0.9 Rhym-CHR KSFM, + 0.6 A/C KYMX, + 0.6 N/T KXJZ; -- 1.0 CHR KDND,
Swings:+ 0.9 @ Rock KRXQ & Country KNCI, + 0.4 @ N/T KFBK, RhymOldies KHYL, RhymCHR KSFM. -- 0.9 Adult Hits KQJK, -- 0.7 Adult Contemporary KYMX, -- 0.7 Hot A/C KZZO.
Swings:+ 1.2 Country KBEB, + 1.1 Rock KRXQ; -- 0.8 RhymCHR KSFM, -- 0.8 AdHits KQJK, -- 0.5 N/T KXJZ.
-- 0.2; 6.1-5.7-5.9, #1 Cume 578,600, leads 2 Rhym-CHRs KSFM 2.4 & KHHM 2.6.
-- 0.3; 6.0-6.1-5.7, #1 Cume 524k, ups RhymCHR CBS KSFM 1.5, Entravsn KHHM 2.9.
Sticks 6.1-6.0-6.1,#1 Cume 503,900, share lead on Ryhthmic CHRs KSFM by 2.1 and KHHM by 2.6.
Gains for 2nd place + 1.0 Entercom Classic Rock KSEG (7.8-8.7-8.8), + 0.8 Entercom Modern Rock KKDO (3.8-4.4-4.6) and + 0.7 CBS Rhythmic CHR KSFM (4.1-4.4-4.8).
CBS RhyCHR KSFM 4.0-4.7-4.3, Entravision RhyCHR KHHM 3.1-3.1-2.5 and iHeart RhyAC KHYL 2.4-2.8-2.6.
CBS: Hot A/C KZZO 4.9-4.7-4.8, RymCHR KSFM 4.2-4.0-4.7 and Country KNCI 4.6-4.1-3.4, tied with Entravision Country KNTY 4.1-3.8-3.4.
ModRock KKDO 4.3-4.5-4.4, CBS KSFM wins RymCHR 4.6-4.3-4.2 over Entravision RymCHR KHHM that grows 2.8-3.5-3.4.