Subsequently, the oocytes were washed to eliminate extra spermatozoa and then cultured overnight in separate dishes, in a drop of potassium Simplex Optimization Medium (KSOM
medium of the treated group was made by addition of [H.sub.2][O.sub.2] to a final concentration of 0 [micro]M, 20 [micro]M, 40 [micro]M, 60 [micro]M, or 80 [micro]M and then preequilibrated them for 30 min before use.
In vitro fertilisation was carried out in drops of KSOM
medium plus 5mg/ml-1 Bovine Serum Albumin (BSA) under mineral oil.
After exposure, embryos were transferred to mPBS + 0.25M sucrose, and after 5min, embryos were washed in mPBS and placed into KSOM
medium droplets for in vitro culture.
She served on the Kentucky Sex Offender Management (KSOM
) task force and took over the training of probation and parole officers tasked with supervising sex offenders.
We obtained human tubal fluid (HTF) and potassium simplex optimized medium (KSOM
) from Chemicon; hyaluronidase, pregnant mare serum gonadotropin, and human chorionic gonadotropin (hCG) from Sigma; fetal bovine serum (FBS) from PAA Laboratories; polydimethylsiloxane (PDMS, Sylgard 184) from Dow Corning Corp; and propidium iodide (PI), SYBR 14, and JC1 from Invitrogen.
Dos de los medios mas empleados en la actualidad son el KSOM
aa y el CR-1 (tabla1).
The manufacturer of KSOM
clampless cutters reports they deliver high metal-removal rates and good surface finishes in steel, stainless steel, aluminum, cast iron, and high-temperature alloys.
Also shown will be new chamfering rings, a spotting drill with built-in chamfering capabilities, and grade KC7315 for its KSEM line of modular carbide drills; KSOM
octagonal insert face mills; and two new coated insert grades, KC5510 and KC5525, which can double productivity in turning high-temperature alloys.
The work in  proposed the usage of Neural Network (NN) and Kohonen Self-Organizing Map (KSOM
) techniques to optimize energy consumption in WSNs.
The oocytes were then placed in KSOM
that contained non-essential amino acids and essential amino acids (KSOM
AA; Specialty Media) supplemented with 1 mg/ml bovine serum albumin (BSA, Sigma, Japan), covered with mineral oil (Sigma, Japan), and stored at 37[degrees]C, in 5% C[O.sub.2] in air (25).
Then, oocytes were activated with Ionomycin Calcium (Sigma) (2.5 |ag/ml) followed by 6-DMAP (Sigma) (2.5 mM) and cultured with droplets of KSOM
medium (Chemicon) in a C[O.sub.2] incubator (Thermo, Forma 311) at 37.5[degrees]C for 4-4.5 days.