By considering standard lines at left and right borders of gel it is possible to recognize which bands are related to KSTI (MW=22 KD) and which one are related to BBI (MW=7.8 KD).
The location of KSTI and BBI in each electropherogram could be estimated by a simple comparison between standard KSTI as a reference electropherogram with inferred results from different peaks.
The highest amount of KSTI was observed at peak 2 G-75 (0.8 mg/ml), peak 3 G-75 (0.47 mg/ml) and peak 2 FC (0.44 mg/ml) respectively.
Otherwise FPLC can separate two kind of trypsin inhibitor from each other (KSTI & BBI) according to molecular weight.