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K2PKimura 2-Parameter
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The tree was constructed by the neighborjoining method using Kimura 2-parameter with gamma distribution (G) and 2,000 bootstrap replications implemented using MEGA 7.
An unweighted pair group method with arithmetic mean (UPGMA) tree of Kimura 2-parameter distances (K2P) amongthe cytochrome oxidase c subunit 1 gene (COI) sequences of Leucothrips collections that are indistinguishable based on adult morphology.
Results from an analysis with a Kimura 2-parameter model yielded distance values ranging from 5.
The tree was created by using the maximum-likelihood method based on the Kimura 2-parameter model with 500 bootstrap replicates from the whole-genome sequence of the displayed sequences.
net/) and a neighbor-joining algorithm with the Kimura 2-parameter model and 1,000 replications of bootstrap analysis.
jp/tools/clustalw/) alignment of P1 nucleotide sequences, followed by maximum-likelihood analysis using the best-fitting Kimura 2-parameter plus gamma distribution model of evolution.
The neighbor-joining method by Kimura 2-parameter distance method was used (1,000 bootstrap replicates).
Neighbor-joining and Kimura 2-parameter methods were implemented by using MEGA version 5.
Phylogenetic analysis of partial cox1 gene sequences was performed by using the neighbor-joining method and the Kimura 2-parameter model in MEGA5 (http://www.
The phylogenies were assessed by applying neighbor-joining and maximumparsimony methods, with the Kimura 2-parameter correction model.
Evolutionary distances were estimated according to the Kimura 2-parameter method (12).