The tree was constructed by the neighborjoining method using Kimura 2-parameter
with gamma distribution (G) and 2,000 bootstrap replications implemented using MEGA 7.
An unweighted pair group method with arithmetic mean (UPGMA) tree of Kimura 2-parameter
distances (K2P) amongthe cytochrome oxidase c subunit 1 gene (COI) sequences of Leucothrips collections that are indistinguishable based on adult morphology.
The genetic distance was calculated for the total population distributed among distinct localities and seasons of the year, using the model of Kimura 2-parameter
Results from an analysis with a Kimura 2-parameter
model yielded distance values ranging from 5.
The tree was created by using the maximum-likelihood method based on the Kimura 2-parameter
model with 500 bootstrap replicates from the whole-genome sequence of the displayed sequences.
net/) and a neighbor-joining algorithm with the Kimura 2-parameter
model and 1,000 replications of bootstrap analysis.
jp/tools/clustalw/) alignment of P1 nucleotide sequences, followed by maximum-likelihood analysis using the best-fitting Kimura 2-parameter
plus gamma distribution model of evolution.
The neighbor-joining method by Kimura 2-parameter
distance method was used (1,000 bootstrap replicates).
Neighbor-joining and Kimura 2-parameter
methods were implemented by using MEGA version 5.
Phylogenetic analysis of partial cox1 gene sequences was performed by using the neighbor-joining method and the Kimura 2-parameter
model in MEGA5 (http://www.
The phylogenies were assessed by applying neighbor-joining and maximumparsimony methods, with the Kimura 2-parameter
Evolutionary distances were estimated according to the Kimura 2-parameter