LABNETLos Alamos National Laboratory (closed circuit television network)
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Samples of the dairy beverage were stirred for 1 min on a vortex stirrer (VX-200, Labnet), and then centrifuged for 20 min at 5000 rpm and 12[grados]C.
En este proceso los ensayos se llevaron a cabo en tubos falcon de 50 ml los cuales se llenaron con 35 g de muestra y se procedio a introducirlos en un bano seco AccuBlock modelo D1200 (Labnet, USA) para controlar la temperatura del proceso.
Ambarees, also known as "Labnet al-Jarra," due to its storage in jars, was once a common dairy product made in most households of the Bekaa Valley.
Inicialmente se sensibilizo la placas con 100 [micro]L/pocillo de la proteina recombinante MSP5r a 2 [micro]g/mL, diluido en buffer de carbonato-bicarbonato 50 mM (pH 9,6) y se incubo la placa a 4 [grados]C en camara humeda (Labnet, 311DS, EUA) por toda la noche.
About 0.5 [micro]L of CNF suspension was dripped using a Labnet micropipette onto a 300-mesh carbon coated formvar copper grid, and the water was allowed to evaporate.
10 [micro]L of the PCR products was then separated on 2% agarose gel (Biopioneer Co., USA) thereafter in 1x TAE running buffer (Biopioneer Co., USA) by electrophoresis at 80 volts (Labnet International, Power station 300) using 2 [micro]L of blue/orange DNA loading dye (6x) (Promega Co., USA) and stained with 0.5 [micro]g/mL ethidium bromide (Life Technologies Co., USA).
As part of manual and semiautomated processes, the reaction vial was heated using an Accublock Digital Drybath heater block (Labnet, Woodbridge, New Jersey, USA) with a modified aluminium adaptor.
Phase separation was performed by centrifugation (Spectrafuge 6C Compact model, Labnet International, USA).
Touchdown PCR was performed on a MultiGene[TM] thermocycler (Labnet International, Inc., USA) using the following cycling conditions: denaturation at 94[degrees]C for 1 minute, followed by a hybridization step using a 0.5[degrees]C decrease for each cycle between 65 and 55[degrees]C, with 10 additional cycles at 55[degrees]C, and the extension step at 72[degrees]C for 3 minutes.
Electrophoresis was carried out at 100 V for 30 minutes using Endoro[TM] Gel XL, Labnet International Inc.
The amplification of the reverse-transcription products was carried out by PCR (1 [micro]l of cDNA, 1.5 mM Mg[Cl.sub.2], 20 [micro]M dNTPs, 10 pmol of primers (Promega, Charbonnieres, France) (Table 1) and 2.5 units of recombinant Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) in a MultiGene cycler (Labnet, Woodbridge, NJ).
Se tomaron entre 50 a 100 mg de tejido conservado, el tejido se lavo con agua destilada tres veces agitando con Vortex (Labnet, USA).