LALNLung-Associated Lymph Node
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W grupach narazanych na stezenia 0,5 mg/[m.sup.3] i 2,5 mg/[m.sup.3] odnotowano zwiekszenie masy pluc, wyrazne wieloogniskowe zapalenie ziarniniakowe, rozproszone zapalenie histiocytarne i neutrofilowe oraz lipoproteinoze wewnatrzpecherzykowa w plucach i zwiazanych z nimi wezlach chlonnych LALN. Ponadto u szczurow narazanych na najwyzsze stezenie wystapila lagodna neutrofilia we krwi.
LALN cells were centrifuged at 230 x g at 4 [degrees]C for 10 min and resuspended in complete medium, which consisted of RPMI 1640 medium supplemented with penicillin and streptomycin (Sigma) and 2% heat-inactivated fetal calf serum (GIBCO-BRL, Gaithersburg, MD).
In the present study, we investigated the response of total lung and LALN enriched DC subsets to IAV infection.
IAV infection can induce gradual increases of the percentage of mDCs in the LALNs of NHPs
We next tested the number of mDCs in LALNs after IAV infection on days 1, 2, 3, 4, 7, and 30, respectively.
Expression levels of HLA-DR, CD80, CD83 and CD86 were also observed in LALNs after IAV infection.