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The SBM (Sanhe Hopefull Grain & Oil Group Co., Ltd., Hebei, China), LCSM (Shandong Futai Grain & Oil Group Co., Ltd., Shandong, China, FG: 302.54 mg/kg), and DRM (Fuzhou Jijia Oils & Fats Co., Ltd., Fujian, China, isothiocyanate: no detected; oxazolidine thioketone: 0.34 mg/g), were obtained from commercial suppliers.
Briefly, four experimental groups were fed a basal diet supplemented with 100.0 g/kg LCSM, 187.0 g/kg LCSM, 148.7 g/kg DRM, or 292.0 g/kg DRM, which corresponded to 50% ([LCSM.sub.50]), 100% ([LCSM.sub.100]), 50% ([DRM.sub.50]), and 100% ([DRM.sub.50]), respectively of the SBM dietary protein replaced by LCSM or DRM.
The replacement of SBM by LCSM or DRM did not affect the LP EW, DFI, FCR, DEM, or AER in weeks 33 to 36 or weeks 37 to 40 (p>0.05).
LCSM is a deep-processed product of CSM with slightly better nutritional value (i.e., CP and total AA) than that of SBM has [8].
The complete replacement of SBM by LCSM (FG, 56.57 mg/ kg) did not lead to yolk discoloration, our study agrees with those of previous studies [2,25].
Cells were then incubated with tubulin-Alex Fluor 488 (0.1 [micro]g/ml) and DAPI (0.1 [micro]g/ml) for 20 min in a dark enclosed space and visualized through LCSM (Carl Zeiss, GER).
Meanwhile, morphologic changes in the nucleus and cytoskeleton of the treated cells were observed under LCSM with DAPI and tubulin Alexa Fluor 488 staining; whereas, the mitochondrial membrane potential was detected with Mito Tracker Red CMXRos staining.
Figure 3 shows LCSM images of ablated craters for laser fluences of 0.8 and 1.6 J/[cm.sup.2] with 500 pulses.