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LIPO-Fat (Prefix)
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To further characterize the inhibitory effect of [Gr-1.sup.+] myeloid cells on lung fibroblasts, RNA was analyzed from lung-purified [Gr-1.sup.+] myeloid cells from Lipo- and Clod Lipo-treated mice at day 5 after bleomycin, and the expression of apoptosis-inducing factors was determined.
All mice were subsequently sacrificed at day 5 after bleomycin challenge, and lungs from D-1 Clod Lipo- or Lipo-treated mice were digested to generate a cellular suspension, and cells were stained with monoclonal antibodies directed against Gr-1, CD11b, CD11c, F4/80, CD3, CD19, [gamma][delta] TCR, or NK 1.1 and analyzed by flow cytometry.
"There have been days when I've received hundreds of calls from people asking about Lipo- Dissolve."