Drugs such as GHB, cocaine, THC, zopiclone, and zolpidem are rapidly eliminated from the blood and even if present at time of driving might have dropped below the method's LLOQ
(and thus been reported as not detected) by the time blood was taken for toxicological analysis.
Eight concentrations of saxagliptin and dapagliflozin ranging from 0.01 to 0.50 [micro]g/mL and 0.05 to 2.00 [micro]g/mL, respectively, were used, which included the LLOQ
, low quality control (LQC), medium quality control (MQC), high quality control (HQC), and upper limit of quantification (ULOQ).
Sensitivity of the method was determined on bases of lower limit of detection (LLOD) and lower limit of quantification (LLOQ
) of studied analytes.
The ADx assay, designed to detect neurogranin truncated at P75, did not detect the calibrator of WashU or the calibrator ofUGot, although, for the latter, lower concentrations at the border of the LLOQ
were detected for all calibrator dilutions.
Application of 100 [micro]L of bleach to wet blood samples resulted in a significant reduction in viral titer to the LLOQ
of the assay (Figure 1, panel B), suggesting that wet blood is less challenging to disinfection than dried blood.
The interference observed at the retention times of analytes and ISTDs in blank plasma lots was compared against mean response of extracted LLOQ
In controlled clinical studies of ZEPATIER, SVR was the primary endpoint defined as HCV RNA less than lower limit of quantification (LLOQ
) at 12 weeks after the cessation of treatment (SVR12).
The primary efficacy end point of this study was a rapid virologic response (RVR), which was defined as an HCV RNA level below the lower limit of quantification, (LLOQ
The lower limit of quantification (LLOQ
) for The Great Plains Laboratory's Glyphosate Test is 0.38 [micro]g/g of creatinine.
Analyte r LLOQ
(ng/g) Recovery (%) DA 0.9969 2.0 94.6 [+ or -] 8.7 DOPAC 0.9982 72.0 93.4 [+ or -] 6.6 HVA 0.9977 50.0 95.3 [+ or -] 7.4 Analyte Intraprecision (RSD %) Interprecision (RSD %) DA 10.3 12.4 DOPAC 8.9 7.5 HVA 7.7 11.3 DA: dopamine; DOPAC: 3,4-dihydroxyphenylacetic acid; HVA: homovanillic acid.
Linearity and LLOQ
. The calibration curves of HPLC method were evaluated by analyzing a series of standard plasma samples at concentration from 0.2 to 200 [micro]g/ml for caffeine and from 0.4 to 400 [micro]g/ml for tolbutamide, chlorzoxazone, and midazolam.
Linearity and LLOQ
. The calibration curves were linear over the concentration range of 2.5-2000 ng/mL.