To verify the transferability of the developed microsatellite loci
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"We have identified 16 genome-wide significant loci
, of which 11 are newly discovered.
In the present study, 10 microsatellite loci
were highly polymorphic, whereas the degrees of diversities were different at different loci
The allelic diversity of Glu-D1 locus greatly affects the bread making quality of wheat as compared to diversity present at others Glu-1 loci
. Moreover, several studies showed that 5+10 subunits combination at Glu-D1 locus provide superior dough quality than 2+12 subunits combination.
DNA extraction was done using Chelex method and the extracted DNA were amplified using 17Y-STRs of AmpFlSTRYfiler[TM] kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a, DYS385b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, and DYS448)  using the following conditions: PCR amplification of the Y filer loci
was performed using 0.5-1 ng of DNA template and the total 25 [micro]L reaction volumes of PCR amplification were used, as recommended by the manufacturer.
Cross species amplification was conducted with two western box turtle (Terrapene ornate) specimens from South Dakota, and no loci
showed cross amplification.
As expected, all 5 loci
showed differences in the number of microsatellite repeats.
Since several loci
were used, each having large amounts of allelic variation, population samples of individual fish need not have been prohibitively large to have generated some confidence in findings.
Adicionalmente, en diferentes estudios se ha demostrado una variabilidad en el nivel del polimorfismo de los loci
MIRU-VNTR que puede atribuirse al origen geografico de la cepa y a diferencias entre los linajes geneticos (9, 6).
This study examined 689 512 STR loci
across human genomes; the 13 STR loci
used by CODIS were thus a very small subset.
These markers are useful for tracing different loci
of Q gene and can also be used in the identification of genetic materials involved in homoeologous group 5 chromosomes.