LOMLLove Of My Life
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Between 1.0L and 1.5L of water or buffer was sterile filtered into the 10L filling bag and then filled into around 400 vials, size lOmL, with a fill volume of 3 mL per vial.
The extract (1 ml) was evaporated to dryness and dissolved in a lOmL volume of solvent [CH.sub.3]CN: DCM=7:3 and out of that a known volume (10[micro]L) was injected into HPLC loop.
It was introduced into the esophagus through the right nostril up to the mediastinum, approximately 135-145cm from the nostril, and only then was the balloon tip inflated with lOmL of air pushed through a syringe.