levels were evaluated in the acidic medium with ferric ion oxidation-xylenol orange (FOX-2) method based on enzymatic oxidation of ferric ion to ferric ion by lipid hydroperoxides.14 Serum SH levels were determined according to Ellman and Hu methods,15,16 as described previously.
As expected, increased levels of gastric LOOH
were confirmed in the ulcerated group treated with vehicle (29.57 [+ or -] 2.99mmol/mg of tissue).
levels were measured with a ferrous ion oxidation-xylenol orange assay.
+ [Fe.sup.3+] [right arrow] LOO* + [Fe.sub.2+] + [H.sup.+]
Blood samples for cytokine, LOOH
, antioxidants, and NE/AAT measurements were taken before surgery, 6h after clamp release or reperfusion of both lower limbs and then at D1, D3, and D5.
levels of groups were examined there was not a significant difference between groups, but after the application raise of LOOH
level in E group is important, and this raise was not in K and GE groups so it can be considered that ginseng has an effect on LOOH
LH + LO[O.sup.*] [right arrow] [L.sup.*] + LOOH
During this process oxidation of lipids generates lipid hydroperoxides (LOOH
), and their formation in membranes disrupts normal cell metabolism, triggering adaptive responses and/or causing cell death (Girotti 1998).
In the propagation step, peroxyl radical is able to abstract H from another fatty acid, resulting lipid hydroperoxide or peroxide (LOOH
Oxidative and antioxidative status were evaluated by measuring serum lipid hydroperoxide (LOOH
) level, total antioxidant status (TAS) and total oxidative status (TOS) (13,14).
The flourescent image analysis for lipid hydroperoxide (LOOH
) by using 3-PeDPP was performed according to the method of Chotimarkorn et al.
Posteriormente se genera un dieno conjugado que reacciona rapidamente con el [O.sub.2] para dar origen a un radical peroxilo ([LOO.sup.*]) (15) y se forma un hidroperoxido lipidico (LOOH
) y un nuevo L' (figura 5) (27).