LPAATLysophosphatidic Acid Acyltransferase
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The range in C12:0 levels in 100 dihaploid plants cotransformed with both the LPAAT and the thioesterase transgenes was from 0 to 67 mol% (Knutzon et al.
Existing high erucic acid rapeseed (HEAR) cultivars have less than 1% C22:1 incorporated into the central position (sn-2) of the glycerol backbone because of the poor affinity of the rapeseed acyltransferase LPAAT for very long chain fatty adds, including C22:1 (Brough et al.
The failure to significantly increase the C22:1 level by engineering LPAAT could be due to a limitation in the acyl-CoA pool in the cytosol, which is required to support high levels of trierucin synthesis (Luhs et al.