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Leucine-rich alpha-2-glycoprotein (LRAG) was selected for relative label-free protein quantification, as there was no coidentification within its spot (spot 20), and the normalized spot volume changed more than 50%.
Western blotting showed an increase in the LRAG plasma level in the RAEB2 patient group--the increase corresponded to approximately 30% when compared with the control group.
LRAG is supposed to be a marker of granulocytic differentiation and a protein involved in neutrophil differentiation with upregulated expression .
The possibility of multiplexing could be favourably utilized for biomarkers like the one proposed in this study (LRAG) that changes in both the plasma level and its modifications; many protein PTMs could be determined during a single analysis of a sample .
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