1,25-Dihydroxyvitamin [D.sub.3] induces expression of the Wnt signaling co-regulator LRP5
via regulatory elements located significantly downstream of the gene's transcriptional start site.
For each sample, target genes (AHRR, ALPPL2, PPP1R15A, SLC24A3, AHRR-C5orf55-EXOC-AS, F2RL3, CPAMD8, HOXA5, SASH1, LRP5
) and the reference gene (ACTB) were amplified in triplicate using TaqMan assays (see Table S2) designed to span exon junctions using Universal PCR Master Mix and run on an ABI 7900HT (Life Technologies).
Interestingly, several genomic regions, such as LRP5
and NEK3 regions, contained multiple significant/suggestive CpG-SNPs, suggesting that multiple neighboring CpG-SNPs may synergistically mediate the DNA methylation and gene expression of the target genes.
Kahn, "Cross-talk between insulin and Wnt signaling in preadipocytes: role of Wnt core-ceptor low density lipoprotein receptor-related protein-5 (LRP5
)," The Journal of Biological Chemistry, vol.
Lrp6 also seems more important for regulation of bone turnover than Lrp5
, as its deletion appears more deleterious to trabecular bone structure and osteoblastic differentiation than that of Lrp5
Autosomal dominant inheritance, due to FZD4, LRP5
, and TSPAN12 gene variations [6-8], is prevalent.
Mutations in LRP5
lead to the development of diabetes and obesity .
norvegicus (provided by NCBI) for dishevelled 2 (Dvl-2), low-density lipoprotein receptor-related protein 5 (LRP5
), glycogen synthase kinase 3 beta (GSK3[beta]), [beta]-catenin, and TCF7L2 and synthesized by TaKaRa (Table 3).
(16-18) Among these genetic variations, lipoprotein receptor related protein 5 (LRP5
) variants have been found to influence the effects of physical activity on spine bone density in males and Wnt signaling in vitro.
Role of the intracellular domains of LRP5
and LRP6 inactivating the Wnt canonical pathway.
One of these RLR-inducible miRNAs, miR-23b, targets very low density lipoprotein receptor (VLDLR) and LDLR-related protein 5 (LRP5
Cripto-1 enhances the canonical Wnt/beta-catenin signaling pathway by binding to LRP5
and LRP6 co-receptors.