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Least square means of body weight changes were -53.89, -24.85 and -37.12 kg/d for LRUP, MRUP and HRUP respectively (Table 4); differences among treatments, blocks and treatments x block were not significant (P > 0.05).
Least square means of NEFA of plasma of the treatments LRUP, MRUP and HRUP were 0.60, 0.55, and 0.48 mM/lit (Table 4).
HRUP = high RUP diet, MRUP = medium RUP diet, LRUP = low RUP diet, MUN = milk urea nitrogen, FM = fish meal, CGM = corn gluten meal, SBM = soybean meal, MP = metabolizable protein, NEB = negative energy balance, NEFA = non esterified fatty acids, [beta]HBA = beta hydroxy butyrate.
Nitrogen balance, whether expressed as g/d or % of N intake was higher (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets (Table 4).
Energy balance was positive in early lactating buffaloes fed HRUP diet, however, it was negative in buffaloes fed MRUP and LRUP diets (Table 6).
Decreased DMI by buffaloes fed LRUP diet was due to the high degradable protein portion of this diet.
Higher DM digestibility in buffaloes fed LRUP diet might be attributed to longer rumen retention time due to decreased DMI.
Fat yield was higher in buffaloes fed HRUP diet than in those fed LRUP and MRUP diets.
Higher N intake in buffaloes fed HRUP diet than in those fed MRUP and LRUP diets was due to their higher DMI.
Moreover, higher BUN values in buffaloes fed MRUP and LRUP diets were because of a higher level of RDP in their diets.
Positive energy balance in buffaloes fed HRUP diet was due to higher energy intake (23.76 Mcal/d) than those fed MRUP (19.68 Mcal/d) and LRUP (17.79 Mcal/d) diets.
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