Parameters pH at Calcium Ashes Proteins 25[degrees]C g/1) g/1) g/1) LSAB
These tissue cross-sections were placed on glasses in a standardized manner and stained with hematoxylin eosin (HE), while the cross-sections for the immunohistochemical stained were placed on silane glasses and colored with (ABC-Avidin Biotin Complex method, LSAB
p53 immunoreactivity was visualized by exposing the cells to HRP LSAB
reagents (Dako) and revealed with diaminobenzidine tetrahydrochloride (DAB).
Dako Cytomation, Membranous (K1494) Glostrup, Denmark E-cadherin 4A2C7 Zymed Laboratories, Membranous (18-0223) San Francisco, California PCNA PC10 Dako Cytomation, Nuclear (M0879) Glostrup, Denmark STAT1 M-22 Santa Cruz Biotechnology, Cytoplasmic (sc-592) Santa Cruz, California and nuclear Antigen Antibody Dilution Retrieval Detection CK7 1:100 P CM ACE EGFR Ready Pk Dako Dako Kit DAB to use E-cadherin Ready WB LSAB
AEC to use PCNA 1:20 P CM AEC STAT1 1:1000 Ventana DAB Abbreviations: CK7, cytokeratin 7; CM, ChemoMate, Dako; DAB, DAB ChemoMate Envision kit, Dako; EGFR, epidermal growth factor receptor; HRP, horseradish peroxidase, rabbit; LSAB
Kit Peroxidase Blocking Solution and AEC Substrate-Chromogen, Dako; P, Protease XXIV, 0.
The ligation of primary antibody to specific CD molecules was detected by LSAB
System (Dako, K0690, USA).
Immunohistochemical studies on the paraffin-embedded sections were performed using the streptavidin-biotin-peroxidase complex method with a Dako LSAB
2 kit (Dako, Carpinteria, California).
From the feedback form obtained from the Anaesthesiologists it was seen that out of 20 participants, 19 of them opined that Whitacre needle was easier to use compared to Quincke needle in performing LSAB
Immunostain visualization was achieved with the standard streptavidin-biotin peroxidase technique (DAKO LSAB
kit, Dako Corporation, Carpinteria, Calif, catalog No.
6) Immunohistochemical staining for L26, CD79a, polyclonal CD3, CD21, and CD35 (Dako) was also carried out to determine the phenotype of Warthin-Finkeldey cells using the LSAB
kit (Dako) and diaminobenzidine as the chromogen in case 1.
2, CK7, and CK20 (Dako Corporation, Carpinteria, Calif) were detected with the Dako LSAB
Para deteccao da reacao imune, foi utilizado o kit LSAB
(d), por 30 minutos.
The immunohistochemical study was performed by the streptavidin-biotin method using an LSAB
(labeled streptavidin-biotin) kit (Dako Corporation, Kyoto, Japan).