The highest percentage of response (88%) was recorded on LSBM fortified with BAP (3.0 [mgl.sup.-1]) and the lowest percentage of response (24%) was recorded on LSBM supplemented with Kn (0.5 [mgl.sup.-1]) (Table 1).
LSBM fortified with different concentrations 2,4-D and NAA were used to induce callus from the vegetative bud explant (Fig.
Both the type of callus obtained from vegetative bud explant (compact creamish white callus and loose brownish friable callus) were subcultured on LSBM fortified with BAP and Kn to study their morphogenetic response.
The highest frequency of response (84%) was observed on LSBM supplemented with BAP (3.5 [mgl.sup.-1]) and the lowest frequency of response (30%) on LSBM fortified with BAP (0.5 [mgl.sup.-1]).
Although diets were originally formulated to contain 12% CP in the low protein diet and 16% CP in the high protein diet, CP contents of the experimental diets actually fed in the present experiment averaged 12.0, 16.2, 12.5 and 15.7% DM for LCGM, HCGM, LSBM and HSBM, respectively.
Although ammonia N for LSBM was numerically higher than that for HCGM (103.40 vs.
As a result, both HCGM and HSBM supplied more RDP than LCGM and LSBM, respectively (Table 2).