These compounds include PGP sparing LTB4
inhibitors, classical and alternative complement inhibitors, anti-histamines, and serotonin inhibitors as examples.
Indeed, B-lymphocytes are able to produce the major 5-LOX metabolites 5-HETE and LTB4
in the presence of exogenous arachidonic acid and oxidative stress (Jakobsson et al.
Consequently, elevated production of leukotriene LTB4
, one of the major products of the 5-LOX pathway, may then exaggerate inflammation (Doi et al.
Inflammatory mediators (TNF-a, IL-6, LTB4
) were determined with commercial kits (Pierce Biotechnology, endogenous human TNF-a; Pierce Biotechnology, endogenous human IL-6, Rockford, USA and Thermo Scientific LTB4
, Waltham, USA) by ELISA.
Plasma PGE2 and LTB4The effects of genistein supplementation on plasma LTB4
and PGE2 are presented in Fig.
1fold) and a corresponding increases in the metabolic activity of eicosanoid LTB4
Consequently, levels of PGE2, LTB4
, and nitric oxide (NO) metabolites were decreased due to the corresponding protein downregulation.
ELISA kits for PGE2, PGF2[alpha], PGI2, PGD2, TXB2, and LTB4
were purchased from Cayman Chemicals Co.
Leukotrienes may be very relevant in this pathology, since LTB4
is the most potent chemotactic agent for neutrophils both, in vitro and in vivo (7).
Sisteinil lokotrienler (LTC4, LTD4, LTE4) ve LTB4
RSV enfeksiyonlu cocuklarda ve fare orneklerinde defalarca nazofaringeal ve trakeobronsiyal salgi larda saptanmislardir (48).
Although both COPD and asthma are characterized by an inflammatory response, the cells and their inflammatory mediators in stable COPD (IL-8, LTB4
, and TNF-[alpha]) are considerably different from those found in asthma (IL-4, IL-5, IL-13).
Leucotrienos: Leucotrienos: Hidrolasa A4, Hidrolasa A4, Fosfolipasa A2, Fosfolipasa A2, LTB4