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In other words, expanded adherent HSPCs exposed to HP (H_M_ NA_HP group) retained the HSPC phenotype (CD[34.sup.+], CD[34.sup.+]CD[38.sup.-], and CD[133.sup.+]CD[38.sup.-]) for the longest time and had the highest clonogenic potential as assessed by CFC and LTC-IC assays.
Cells cultured for 4 days under different conditions were cultured for 6 additional weeks in long-term culture-initiating cell (LTC-IC) assays, and colony-forming cell (CFC) assays were then performed (n = 3, * p <0.05).
Conclusion: We found that number of LTC-IC obtained from CD34+ cord blood cells were relatively low in numbers when compared to previously reported bone marrow CD34+ cells.
Key words: Stem cell, cord blood, LTC-IC, limiting dilution, CFU assay, cytometry
Anahtar kelimeler: Kok hucre, kordon kani, LTC-IC, kisith dilusyon, CFU kulturu, akan hucre olcer
The long-term culture-initiating cells (LTC-IC) are the most primitive human hematopoietic cells detectable by in vitro functional assays.
The LDA method for LTC-IC was the method of choice in our study to achieve more efficient results.
The type of the feeder layer, cell concentration, presence of accessory cells, change of culture media, and additional growth factors in the media are important for the proliferation of LTC-IC in culture media [31.
Cells obtained through LTC-IC are the most primitive cells that can be analyzed by in vitro functional assays.
LTC-IC absolute counts with LDA were evaluated as described previously (10-12).
El factor de transcripcion HOXB4 es el que mas se ha estudiado, sus niveles de expresion se encuentran elevados en celulas de medula osea CD34+ CD38-/[loCD45RA.sup.-][CD71.sup.-] que contienen gran cantidad de celulas iniciadoras de cultivo a largo plazo (LTC-ICs), pero no se ha encontrado HOXB4 en la mayoria de poblaciones de celulas progenitoras adultas (72).
Caption: Figure 4: MSCs from UCB and PL favor the formation of LTC-ICs. Fold increases in the numbers of LTC-ICs on day 14 of culture.
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- LTC4 transporter