A total of 62 and 80 enzyme-producing strains were isolated from ATMR and LTMR silages, respectively.
The effects of pH on the amylase and hemicellulase activities of microbes isolated from ATMR and LTMR silages during ensiling are shown in Table 6.
The differences in initial chemical and microbial compositions between ATMR and LTMR may lead to different variation trends in some fermentation qualities, microbial and chemical compositions parameters between two types of TMR silages during ensiling, which could be responsible for the significant interactions between days of ensiling and type of TMR observed in this study.
In this study, eight species that produced amylase or hemicellulase were isolated from ATMR, LTMR, and their silages.
xylanexedens were the main hemicellulase-producing microbes in LTMR and its silages.
xylanexedens are the main amylase-producing microbes during the early stage of ensiling in ATMR and LTMR silages, respectively.
A different pattern was observed in TMR silages: the LAB populations showed a slight downward trend during the first 120 h in ATMR silage and 72 h in LTMR silage, regardless of ensiling time, and then increased gradually until they were stable.
In LTMR silage, pH began to rise rapidly at 120 h after a stable period.
The concentration of acetic acid gradually decreased in ATMR silage, while it peaked at 24 h and then decreased in LTMR silage (Figures 2 and 3).
acidilactici lost their dominant position in the later stage of aerobic deterioration in ATMR and LTMR, respectively, P.
05) were observed between 28 and 56 d for LTMR silage.