In our paper, we choose [L.sub.S0], [L.sub.S1], and Ltq
operators to investigate anomalous quartic couplings.
The separation and identification of digested protein were conducted by a Finnigan linear trap quadrupole (LTQ
) mass spectrometer (Thermo Finnigan, San Jose, CA) coupling with a high performance liquid chromatography (HPLC) system (Thermo Finnigan) according to Stastna et al.
The resulting tryptic peptides were separated and analyzed using reversed-phase capillary high-performance liquid chromatography directly coupled to a Thermo LTQ
Orbitrap mass spectrometer using published procedure described by Zuo et al.
MS was performed on a Thermo Fisher (Thermo Fisher Scientific, Franklin, MA, USA) LTQ
Orbitrap XL hybrid mass spectrometer.
Customers using the Exactive, Q Exactive and LTQ
Orbitrap Hybrid FT series of mass spectrometers will be able to incorporate ZipChip to enhance their analytical capabilities.
Proteins were digested overnight at 37 [degrees]C in 10 ng/[micro]l trypsin, and the resultant peptides were fractionated on a reversed-phase column and introduced directly onto a Thermo-Finnigan LTQ
linear ion trap mass spectrometer (Thermo Fisher Scientific) via electrospray ionization.
Intrigues and intricacies of the biosynthetic pathways for the enzymatic quinocofactors: PQQ, TTQ, CTQ, TPQ, and LTQ
mass spectrometer and TurboSequest Bioworks were from Thermo Finnigan (US).
La muestra seca se reconstituyo con 0.2 ml de metanol al 50% y se analizo por HPLC-esi(+)MS utilizando un espectrometro de masas Velos Pro LTQ
(Thermo Scientific, USA) acoplado a un cromatografo Agilent 1200 (Agilent Technologies, USA) con una columna Betasil C18 (100 x 2.1 mm).
Fractions containing tryptic peptides were dissolved in aqueous 25% v/v ACN and 1% v/v formic acid and these were resolved and ionized by using nanoflow HPLC (EasynLC II, Thermo Scientific, Waltham, MA) coupled to the LTQ
XL-Orbitrap hybrid mass spectrometer (Thermo Scientific).
The HRMS of the products were measured on a LTQ
Orbitrap (Thermo Electron) spectrometer (positive ionization, static nanospray, boron silicate emitters Proxeon, resolution 100 000 at m/z 400, external calibration), using acetonitrile as the solvent.
Samples were injected onto a Thermo Scientific Accela HPLC system coupled to a Thermo Scientific LTQ
Orbitrap Discovery Mass Spectrometer (Thermo Fisher Scientific, Waltham, USA).