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Optimization of the system with BBD involves four steps: (i) identifying the output variable to optimize, (ii) identifying and selecting factors and levels that affect the LVSS system, (iii) data analysis and fitting of the surface response model, and (iv) confirmation under the optimal conditions obtained.
For LVSS, the output variable selected is the sum of the enrichment factors of the four TCs.
In this study a new method combining the LVSS technique and high-efficiency CE was developed in order to analyze and determine three compounds in Flos Farfarae .
There are three main steps in the LVSS method [31-33]: first, the capillary was filled with the running buffer; then the standard or sample was injected by hydrodynamic injection at the pressure of 0.5 psi in short time (about 4 seconds); second, when the sample solution flows into the capillary after a certain length, reversing polarity voltage (about 25 kV) was applied; third, the sample was stacked at the interface between the buffer and the sample zone.
LVSS was applied to increase the analyte concentration prior to their separation.
Finally LVSS was used to preconcentrate the extract obtained from real samples (bovine milk) after the different sample treatments were applied.
Says Paroshen Naidoo of Util Labs: “We installed several of our latest LVSS devices at the temporary supply points at the COP17 Climate Change Response Expo, which measured and stored details of the consumption per phase in real time.
The present study aimed to compare inhaler technique and preference with MDIs and LVSs in inhaler-naive elderly patients with reversible obstructive airways disease.
Thirty patients were provided with LVSs (including MDIs) and ten with MDIs only.
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