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Meanwhile, the FSHR mRNA levels of SYF and LWF were lower compared with that of F3 granulosa layer.
However, LHR mRNA abundance remained low in both SYF and LWF. In the daidzein-treated group, LHR mRNA expression in the granulosa layers of PRFs was significantly higher than that of the control group (Figures 2B and 4; p<0.05).
Nevertheless, the relative abundance of P450arom mRNA in SYF and LWF was higher than that of PRFs.
We found that daidzein treatment markedly increased the numbers of SYF and LWF compared with the control in white silky fowl after peak laying.
The FSHR mRNA level of SYF was higher than that of LWF. These results were consistent with previous reports that the granulosa layer of SYF and F6 to F3 are the primary targets for FSH (Zhang et al., 1997; Calvo and Bahr, 1983).
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