Specifically, we identified two cell lines--MeWo and Mel1700--that consistently displayed differences in (a) susceptibility to productive infection, (b) timing and robustness of virus replication, (c) expression of KSHV latency-associated nuclear antigen, LANA, (d) replication phase-specific expression of viral genes, and (e) susceptibility to virus-induced perturbation of the anti-inflammatory melanogenic response.
For example, during infection with Epstein-Barr virus (EBV), a gammaherpesvirus closely related to KSHV, at least eight different transcripts of the EBV latency-associated nuclear antigen (i.e., EBNA-1, 2, 3A, 3B, 3C, 4, 5, 6), are expressed to control various states of the EBV latency program .
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Nishimura et al., "Accumulation of heterochromatin components on the terminal repeat sequence of Kaposi's sarcoma-associated herpesvirus mediated by the latency-associated nuclear antigen," Journal of Virology, vol.
Choe, "Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) binds ATF4/CREB2 and inhibits its transcriptional activation activity," Journal of General Virology, vol.
Lieberman, "Acetylation of the latency-associated nuclear antigen regulates repression of Kaposi's sarcoma-associated herpesvirus lytic transcription," Journal of Virology, vol.