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References in periodicals archive ?
However, ligand binding assays (LBAs) are much more affected by the heterogeneity of disease-state matrices than small molecule drugs.
(A) Ligand binding assay for GPER in SkBr3 cells exposed to increasing concentrations of E2 or atrazine (Atr) for 2 hr.
Testing performed on the same blocks using a previously validated ligand binding assay 3.
The DAR can make a difference to the performance of an assay in multiple ways, depending on the drug substance and the reagents chosen for the ligand binding assay.
We used rosiglitazone as a positive control in the ligand binding assay. As shown in Table 1, the [IC.sub.50] of rosiglitazone was 0.23 [micro]M; the FP range was > 120 mP, indicating a good dynamic range for the dose response.
Our area of interest is ligand binding assays in which the analyte is quantified, directly or indirectly, on the basis of its specific affinity for a chemically modified solid material.
Due to the possible antiosteoporotic effects of Ecd we tested its estrogenicity utilizing ligand binding assays, the uterotrophy assay and the effects on serum LH levels.
The AhR ligand binding assay was repeated 3 times with three replicates per test compound while cell culture experiments were conducted trifold with eight replicates per concentration of tested sample.
Ligand binding assays were carried out according to the procedure described by Barturen and Garcia-Sevilla (1992) with slight modifications.
These issues were addressed using purified L-FABP, fluorescent ligand binding assays, circular dichroism (CD), and cultured primary hepatocytes from wild-type and L-FABP (-/-) null mice.
Both ligand binding assays (LBA) (2) and mass spectrometry (MS) methods are widely used in routine clinical analysis.
Viswanathan, et al., "Quantitative Bioanalytical Methods Validation and Implementation: Best Practices for Chromatographic and Ligand Binding Assays," AAPS J.