After 24 h, the liver, kidney, spleen
, muscle, heart, brain, intestine, and gill of each fish from five randomly chosen individuals in each group were immediately frozen in liquid nitrogen and stored at -80AdegC until RNA extraction.
Bacteriological samples of liver, kidney, spleen
and blood were streaked onto Tryptic Soy Agar (TSA) and the plates were incubated at 22-23[degrees]C for 48-72 hours.
For sampling, the tissues of the brain, trachea, lung, caecal tonsil, liver, kidney, spleen
, heart, proventriculus, intestine, and thymus were fixed in 10% buffered formalin.
In separate experiments, animals were sacrificed by an overdose of sodium pentobarbital, and their lung, heart, liver, kidney, spleen
, and whole brain were quickly collected and rinsed with ice-cold PBS (pH 7.4) before homogenization in 50 mM Tris buffer, pH 7.4, containing 400 mM NaCl and 0.5% Triton X-100 at 4[degrees]C .
At 21 days post-infection, after disease severity scoring, samples of brain, lung, spinal and hind-limb muscle, liver, kidney, spleen
, heart, and intestine tissue were examined.
Other organs that had significant Zn concentration were liver, kidney, spleen
, and pancreas; liver, spleen, and pancreas are closely associated with Zn metabolism while kidney is related with Zn excretion.
Histological analysis of various radiation-induced tissue damage was performed on all organs collected (heart, lung, liver, kidney, spleen
, stomach, whole intestine, muscle and skin of the posterior femur of right leg, and the brain).
In subacute toxicity study, rats received OSE by gavage at the doses of 200, 400, and 800mg/kg/day (n = 6/group/sex) for 28 days, and biochemical, hematological, and histopathological changes in tissues (liver, kidney, spleen
, heart, and testis/ovary) were determined.
The liver, kidney, spleen
, and heart had multifocal infiltrates of lymphocytes and plasma cells.
Parts of frozen brain, lung, liver, kidney, spleen
, and duodenum content were ground separately, and virus isolation testing was conducted by using embryonated egg injection.
The dams were anesthetized with diethyl ether and then sacrificed by exsanguination; the body weights and organ weights (liver, kidney, spleen
, and thymus) were then recorded.