In present study, considering the health concerns of both transgenic animals and humans, we introduced the loxP
sites to flank the selectable marker genes, which will allow the removal of antibiotic-resistant marker genes by cre-mediated recombination in the future study .
Soarecii CREBCamKCre7 au fost generati prin incrucisarea soarecilor CREBloxP/loxP
cu mutanti exprimand Cre-recombinaza de tip postnatal sub controlul promotorului CamKII[alpha]; soarecii CREBNesCre au fost generati prin incrucisarea soarecilor CREBloxP/loxP
cu mutanti, cu o transgena Crerecombinaza stimulata de nestina (Nes); soarecii CREBloxP/loxP
au fost obtinuti prin flancarea exonului 10 al CREB cu siturile loxP
(Balschun et al, 2003).
1, Cre-mediated recombination between lox75 and loxP
should generate a loxP
"footprint" in the donor locus.
When Cre encounters two loxP sites that scientists have inserted into a mouse chromosome, the enzyme cuts out the intervening DNA (SN: 7/9/94, p.
Since every cell contains two copies of most chromosomes, the loxP insertions sometimes land on different copies of a particular chromosome.
The 34-nucleotide loxP
sequences were originally derived from bacteriophage P1 .
Cre recombinase recognizes loxP
site and mediate loxP
flanked gene mutations.
These sites, known as loxP
sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.
Excision and circularization of the plasmid DNA from the linear bacteriophage DNA is readily achieved by a process involving in vivo excision using Cre recombinase-mediated sire-specific recombination at two loxP
sites flanking the embedded plasmid.
In front of the start codon located in exon 1, the inserted STOP-tetO cassette contained the following sequences in order: loxP
, FRT, a neomycin resistant gene, STOP, FRT, tetO, and loxP
Cre recombinase recognizes and cuts a short bacterial DNA segment called a loxP
TM] line (Figure 4A line 1) can be used to excise DNA sequence flanked by two loxP
sites (Figure 4A line 2).