To deregulate PI3K/Akt and canonic Wnt signaling in the mouse ovarian surface epithelium, genetically engineered mice with loxP
sites in selected introns of the Pten and Apc genes were crossbred so that both copies of both genes contained the appropriate loxP
When Cre encounters two loxP
sites that scientists have inserted into a mouse chromosome, the enzyme cuts out the intervening DNA (SN: 7/9/94, p.
coli expression vector containing loxP
sites to facilitate the removal of the kanamycin marker.
TM] line (Figure 4A line 1) can be used to excise DNA sequence flanked by two loxP
sites (Figure 4A line 2).
In the US, patents were granted on chemisynthetic libraries, use of self-splicing introns with the loxP
dual combinatorial system, and on CAT's ProxiMol(R) technology.