In present study, considering the health concerns of both transgenic animals and humans, we introduced the loxP
sites to flank the selectable marker genes, which will allow the removal of antibiotic-resistant marker genes by cre-mediated recombination in the future study .
Soarecii CREBCamKCre7 au fost generati prin incrucisarea soarecilor CREBloxP/loxP cu mutanti exprimand Cre-recombinaza de tip postnatal sub controlul promotorului CamKII[alpha]; soarecii CREBNesCre au fost generati prin incrucisarea soarecilor CREBloxP/loxP cu mutanti, cu o transgena Crerecombinaza stimulata de nestina (Nes); soarecii CREBloxP/loxP au fost obtinuti prin flancarea exonului 10 al CREB cu siturile loxP
(Balschun et al, 2003).
An additional method for cell lineage tracing is genetic recombination by use of Crecombinase and its target loxP
The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP
The strategy relies on the use of two different transgenic lines, a target line that contains a target lox76 site and expresses cre gene, and a donor line that contains a DNA construct flanked by lox75 and loxP
. This construct consists of a promoterless neomycin phosphotransferase (npt) gene and a [beta]-glucuronidase (gusA) gene.
The enzyme, Cre recombinase, or simply Cre, recognizes short viral DNA sequences called loxP
Onkojenik hedef geni, ekspresyonunu onleyen iki loxP
bolgesi-durdurma sinyali-arasina yerlestirilen bir fare ile Cre-rekombinaz eksprese eden bir fare susu ile caprazlanir (13).
A mouse line with SMC-specific homozygous deletion of the STAT5a/b locus had been previously produced by mating [STAT5a/b.sup.fl/fl] mice carrying loxP
inserts bracketing the entire STAT5a/b locus in C57BL/6:129J mice provided by Dr.
used CD11c-Cre LoxP
system to engineer a TNIP1 DC-specific knockout .
Despite the various uses of inducible transgenes and the Cre/ loxP
system in transgenic mice, there are few reports on the production of transgenic poultry using these systems.
The selection markers were: PGK, mouse phosphoglycerate kinase promoter; NEO, neomycin phosphotransferase; HPRT, mouse hypoxanthine phosphoribosyltransferase; TK, human herpes simplex virus type 1 thymidine kinase promoter; MT, mouse metallothionein promoter; loxP
, a 34 bp recombination site from phage P1.
The targeting strategy is flanking exon 4 of the mouse SNAP-25 gene with two loxP