M-LACMulti-Lectin Affinity Chromatography
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Our M-LAC system displayed ease of implementation and high throughput, enabling parallel sample processing and rapid capture and elution, e.g., 8 h for a 15-sample set.
Our results indicate that isolation of the serum glycoproteome by M-LAC coupled with LC-MS/MS and the use of GO associations facilitates characterization of a large panel of candidate markers, which can then be evaluated in a particular patient population.
[4] Nonstandard abbreviations: M-LAC, multilectin affinity chromatography; DCIS, ductal carcinoma in situ; IC, invasive breast cancer; ACN, acetonitrile; MS, mass spectrometry; MS/MS, tandem mass spectrometry; EIC, extracted ion chromatogram; GO, gene ontology; LC, liquid chromatography.