The assays shall be developed on Bruker's rapifleX MALDI
Tissuetyper using Protea's proprietary histology guided mass spectrometry workflows and expertise.
We will be better able to develop protocols using AMRI's novel, label-free screening assays and extend the utility of the MALDI
PharmaPulse technology to complex cell-based assays for the identification of potential new drugs to meet a variety of unmet needs.
The combination of SCiLS software and MALDI
imaging systems like the Bruker rapifleX high-resolution MALDI
-TOF(/TOF), and the solariX XR extreme-resolution MALDI
-FTMS, expand the scope of MSI from life-science research to pharmaceutical applications with the Molecular Drug Imager Solution, and to translational pathology research with the MALDI
Biotyper provides fast, highly accurate and cost-effective microbial identification with very broad species coverage, based on proteomic fingerprinting.
TOF MS has largely reduced the need of many alternative identification assays.
Innovative technology incorporated into the MALDI
-7090, such as ASDF- Axial Spatial Distribution Focusing, delivers market-leading high-resolution MALDI
MS/MS for accurate compound characterization.
The program generates high quality reconstituted MALDI
images in 2D and 3D based on the m/z value of target compounds.
During the collaboration period of one year, each bacterial isolate received by SBRL will be tested using the MALDI
Biotyper, traditional phenotypic and 16S ribosomal RNA gene sequencing to validate the results.
Among specific topics are the electrochemistry of the electrospray ion source, a comparison of MALDI
matrices, Fourier transform ion cyclotron resonance and magnetic sector analyzers for ESI and MALDI
, ion activation methods for tandem mass spectrometry, and carbohydrate analysis.
Samples are prepared using a microtome and are then coated with matrix for direct analysis via a MALDI
interface, which allows the retention of spatial information.
The Ekspot spotter/fraction collector deposits fractions eluted from the NanoLC onto a MALDI
plate, while a built-in pump automatically adds the matrix.
8-10) In MALDI
the material to be analyzed (the analyte) is mixed with an analysis aid (the matrix) which is easily volatilized by the appropriate laser light.