Use of MALDITOF
mass spectrometry for identification of bacteria that are difficult to culture.
tularensis by MALDITOF
MS exhibited good correlation with identification obtained by molecular methods.
1]H gHSQC NMR spectroscopy and MALDITOF
"mapping" performed on specific enzymic digest mixtures of a protein/ glycoprotein, or on-line ES-MS, provide reliable methods of confirming the overall amino-acid structure and can pinpoint areas for further investigation.
The investigators used surface-enhanced laser desorption/ionization TOF (SELDI-TOF), a variation on MALDITOF
that incorporates protein microarrays and is particularly well suited to detecting patterns of proteins in samples.
Varios metodos experimentales han sido usados, siendo los mas frecuentes la Cromatografia de Permeacion en Gel (GPC), Osmometria de presion de Vapor (VPO) y Tecnicas de Espectrometria de Masas, como MALDITOF
In this issue of Clinical Chemistry, these investigators report their attempt to use MALDITOF
MS as a clinical test to screen for the presence of M-components, identify the heavy and light chain, and quantify the concentration of intact monoclonal immunoglobulin.
Unlike molecular biological methods such as fluorescence in situ hybridization (FISH) and realtime polymerase chain reaction (RT-PCR), MALDITOF
MS requires no species-specific probes, considerably reducing the identification cost (11-29).
The Microbiology Department has implemented the Bruker MALDITOF
Identification of both isolates was confirmed with Bruker MALDITOF
, using Biotyper FlexControl 3.
Abbreviations: TLC: thin-layer chromatography; HPTLC plate: high performance thin-layer chromatography plate; MALDITOF
MS: matrix assisted laser desorption ionization time of flight mass spectrometry; PVDF: polyvinylidene difluoride; MAb: monoclonal antibody; HPLC: high performance liquid chromatography; HSE cell: hepatic sinusoidal endothelial cell; GBS: Guillain-Barre syndrome.
IP, DiGE, ICAT, SELDI, MALDITOF
, Protein/antibody array, etc.