SCV performed extremely well on standard maps --and most especially on map3 and map4, likely due to its being trained on these map types.
Looking at the per-map results, we see that in this partially observable setting, no bot managed to win a single game in the very large map4, so all games there ended in a tie.
Then, incubation with MAP4 antibody (Proteintech, Rosemont, IL, US) diluted at 1: 4000 in TBS containing 0.5% BSA was carried out at 4[degrees]C overnight followed by further washing with buffer to remove unbound antibody.
MAP4 staining was mainly localized in the cytoplasm.
The primers for MAP4 and [beta]-actin were as follows: forward, 5' -CCGGGAACTCAGAGTCAAAGA-3' and reverse, 5' -CTCCATGACCACCATTGGCT-3' (MAP4); forward, 5'-AGCGAGCATCCCCCAAAGTT-3' and reverse, 5'-GGGCACGAAGGCTCATCATT-3' ([beta]-actin).
After blocking with 5% nonfat milk in TBS-Tween-20 for 1 h at room temperature, the membranes were incubated with primary antibodies targeting [beta]-actin (Beyotime Biotechnology, Nantong, Jiangsu, China) and MAP4 (Proteintech, Rosemont, IL, US).
The chi-square test or Fisher's exact test was carried out to evaluate the relationship between MAP4 expression and the clinicopathological variables.
(a) Probe set ID Gene symbol Location 200800_s_at HSPA1A 6p21.3 242904_x_at MGC8721 8p12 213281_at JUN 1p32-p31 229264_at FLJ39739 237510_at MYNN 3q26.31 229054_at FLJ39739 202732_at PKIG 20g12-q13.1 229872_s_at KIAA0493 8q24.13 230574_at 224495_at MGC10744 17p13.1 243_g_at MAP4
3p21 244741_s_at MGC9913 19q13.43 221419_s_at 219503_s_at FLJ11036 3p25.1 240406_at USP16 21q22.11 241749_at 9q31.1 228932_at 227667_at 239063_at 236509_at 200655_s_at CALM1 14q24-q31 221384_at UCP1 4q28-q31 203834_s_at TGOLN2 2p11.2 225122_at RNF31 14q11.2 229975_at Probe set ID Gene description 200800_s_at heat shock 70 kDa protein 1A 242904_x_at hypothetical protein MGC8721 213281_at v-jun sarcoma virus 17 oncogene homolog (avian) (b) 229264_at FLJ39739 protein (M.
To investigate the in vivo functions of MAP4, we prepared stably transfected clonal mouse [L.sup.tk-] cell lines expressing either full-length MAP4 (L-MAP4 cells) or its MT-binding domain (L-MTB cells); see Figure 1.
The [L.sup.tk-] cell lines we have generated, which inducibly overexpress different levels and domains of MAP4, have allowed us to address further questions about the functional interactions of MAP4 in vivo.
In both high- and low-expressing LMAP4 and L-MTB cells, movements of organelles were severely decreased, as compared with L-MOCK or [L.sup.tk]-cells, and the inhibition was greater in the lines that expressed higher levels of MAP4 or MTB.
To elucidate the types of transport events altered by overexpression of MAP4 or MTB, we used fluorescent markers to perform several assays of cell-sorting functions.