Production of ROS by LPS-Induced RAW264.7 Cells Treated with MBHA. It has been reported that mitochondrial ROS molecules act to trigger the production of inflammatory cytokines [26, 27].
Effect of MBHA on mRNA Expression of IL-1, IL-6, and COX-2.
Effect of MBHA on IL-1, IL-6, and TNF Production from LPS-Induced RAW264.7.
The MBHA compounds are a class of molecules which have shown high anticancer activity against a variety of cancer cell lines [20-23].
It was reported that MBHA compounds demonstrated a poor cytotoxic effect in normal cells and these compounds have close relationship between inflammation and cancer .
Initially, we examined the effects of MBHA compounds on cell viability in RAW264.7 macrophage cells.
These results suggest that MBHA compounds can exert an anti-inflammatory effect.
The adducts A2CN, A3CN, and A4CN incubated with LPS almost completely inhibited ROS production in RAW264.7 cells, even at low concentration experienced, and this result together with the reduced synthesis of NO provoked by such compounds, clearly demonstrate the antiredox potential of MBHA molecules.
For a more clear understanding of A2CN, A3CN, and A4CN effects, we evaluated whether the change in the levels of mRNA for the genes IL-1[beta] and IL-6 in RAW264.7 cells stimulated with LPS and treated with 10 [micro]M of all MBHA might occur.
Once it has recently demonstrated the action of mitochondrial ROS (MTROS) as signaling molecules to trigger the production of proinflammatory cytokines such as IL-6 and TNF-[alpha] , we sought to demonstrate the action of MBHA in the production of these inflammatory cytokines in RAW264.7 cells stimulated with LPS.