MBOFModified Basket of Factors (bus fare adjustment; Hong Kong)
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NoLimits 2 runs under Windows 8/7/Vista/XP and requires a 2.0 GHz Dual Core CPU with 2GB of RAM, DirectX 9.0c, 512 MBof video RAM, and 1 GB of hard drive space.
Foram submetidas a extracao de acidos nucleicos, utilizando kit comercial, e posteriormente a PCR com primers MGSO e GPO3 para amplificacao de Mycoplasma spp.As amostras positivas, foram entao submetidas a PCR com os primers mBOR e mBOF, para amplificacao de Mycoplasma bovis.