A representative from DBSI had entered into discussions soliciting the sale of Pennzoil-Quaker State Company bonds with a member of MBOI
. (99) This discussion was conducted over an instant messaging service provider, Bloomberg Messaging System; at first, MBOI
rejected the trade proposal, but approximately ten minutes after the initial online discussion, MBOI
initiated a new instant message inquiring as to "whether the price originally quoted...
"We commend the foresight of our leaders in making this crucial commitment to eliminating malaria" added Dr Mboi
pylori strains with the restriction enzymes HhaI and MboI
By this method, the 16S rDNA sequence was amplified by using PCR, followed by restriction of the amplified fragment by 5 restriction enzymes: CfoI, AluI, MboI
, RsaI, and MspI.
All ITS obtained were analysed by restriction fragment length polymorphism (RFLP) to discriminate the Acinetobacter baumannii specie using the MboI
enzyme which, according to in silico results, led to differentiating such specie from the others in the complex.
Also worrying is the rise of money politics at the local level; one former governor from the Soeharto era has pointed out that 'a candidate without money will almost surely not be elected' (Mboi
(Lamiaceae) llamada "mboi
chini ka'a" es decir, la "hierba de la cascabel".
The restriction enzymes AluI, CfoI, MboI
, RsaI and MspI (Gibco BRL, Paisley, UK) were used in separate reactions to digest the amplimers, according to the manufactures' instructions.
sobrinus se utilizaron las enzimas MboI
(Fermentas, Lituania) y MspI (Amersham Biosciences, Suecia), respectivamente, siguiendo las instrucciones del fabricante.
PCR products were digested with different restriction endonucleases (DdeI, MboI
, MboII, and MseI.).
Following amplification, PCR products were digested in seven reactions with DraI, HaeIII, NlaIII, NcoI, AseI, HpaII and MboI
. Restriction enzyme incubations were run in 7 ul volume consisting of 0.7 ul of the appropriate 10x buffer, 2-4 U of restriction enzyme and 5 ul of the PCR product.
Using a previously described procedure (18), we genotyped all participants by PCR amplification of exon 7 with the primers 5'-CATGAGGCTCAGCCCCAGAAC-3' (sense) and 5'AGTCAATCCCTTTGGTGCTCAC-3' (antisense) followed by MboI
restriction enzyme digestion for 16 h at 37 [degrees]C.