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MCF-7 (ATCC[R] HTB-22[TM]) cells were purchased from the American Type Culture Collection (Manassas, USA).
The cytotoxic effect of stigmasterol and stigmasterol derivatives were assayed with 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) method in three human cancer cell lines (MCF-7, A549 and HepG2) with some modification 25% trypsin would be digested during the phase of cells logarithmic growth and then made into cell suspension, the cell density was determined as:
For MCF-7 experiments, a human breast cancer cell line (DSMZ, Germany) was used.
Breast cancer MCF-7 cells were obtained from American Type Culture Collection (ATCC, USA).
Luminal A BCa cell lines (BT474 and MCF-7) and TNBC cell lines (MDA-MB-231 and MDA-MB-468) were purchased from ATCC and cultured in DMEM media (Gibco/Life Technologies) supplemented with 10% FBS and 1% antibiotic/antimycotic.
A number of changes were observed in both MCF-7 and T47D cells following the development of tamoxifen resistance as the morphology of the cells was significantly altered, with the tamoxifen-resistant MCF-(TamR) and T47D (TamR) cells, respectively, growing in a more scattered fashion and had a more angular appearance which displayed the longer processes previously observed in culture compared to its corresponding wild-type which generally grew in more clearly defined colonies (Figure 3).
The human breast adenocarcinoma cell line (MCF-7) was procured from National Centre for Cell Science, Pune, India.
In order to better understand the potential involvement of leptin in the expression of adiponectin receptors, AdipoR1 and AdipoR2, in the cells of breast cancer, the main objective of this research was to demonstrate that AdipoR1 and AdipoR2 were expressed in MCF-7 and HCC1937 breast cancer cell lines, as well as to determine and analyze the expression of these receptors modulated by differential concentrations of leptin .
Stable NLRP1-expressing MCF-7 cells or MCF-7 cells transfected with vector alone (1 x [10.sup.7] cells in 150 [micro]l PBS) were subcutaneously injected, respectively, into the right and separately into the left dorsal flank of each mouse.
MCF-7 cells were harvested by the use of ice-cold RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) and protein concentration was determined using the BCA protein quantification kit (Beyotime Biotechnology, Shanghai, China); western blotting was performed as previously described .
Human breast cancer cells lines, MDA-MB-231 and MCF-7, were obtained from the Korean Cell Line Bank (Seoul, Korea) and grown in DMEM medium supplemented with 10% FBS at 37[degrees] C under a humidified atmosphere of 5% C[O.sub.2].
MCF-7 (Michigan Cancer Foundation-7) is a breast cancer cell line isolated in 1970 that has been used in original research published in more than 23,000 scientific articles.
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