The human MCF-7
breast cancer cells were purchased from National Cell Bank of Iran (Pasteur Institute, Iran).
cells were from American Type Culture Collection (ATCC, Manassas, VA, USA).
human breast cancer cell line was obtained from the Cell Bank of Shanghai, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China).
9] M) induce growth kinetics and micronucleus formation in MCF-7
breast cancer cells.
cells were purchased from Shanghai Institute of Cell Biology.
On the basis of these observations, we reasoned that the MCF-7
human breast cancer cell proliferation (the E-Screen) assay (Soto et al.
A paclitaxel resistant cell line (MCF-7
/PTX) was established from its isogenetic cell line MCF-7
by increasing the concentration paclitaxel in the culture medium in a stepwise manner over a period of 12 months, the condition of culture was same as MCF-7
breast cancer cells and HepG2 liver cancer cells were purchased from the American Type Culture Collection (Manassas, VA).
Chemical structure of mollugin and characterization of MCF-7
We also examined the estrogenic and antiestrogenic activities using estrogen-responsive element (ERE)-luciferase reporter-transfected human breast cancer cell line MCF-7
However, anti-metastasis effect of curcumin in MCF-7
cells has not been reported and the molecular mechanisms by which curcumin suppressed these effects are not well-known.
For this report, we examined the estrogenic activities of styrene oligomers in the presence or absence of a liver microsomal metabolic system using a yeast estrogen screening (YES) assay and an estrogen-responsive element (ERE)-luciferase reporter assay in the estrogen-responsive human breast cancer cell line MCF-7