Fluconazole (FLC) and micafungin (MCFG) 0.5 mg/mL solution were prepared dissolving the agent in endotoxin free water.
A dilution of the oil was prepared (0.01219 to 12.48 mg/mL), together with a series of two fold serial dilutions of FLC in the 0.06-64 [micro]g/mL range and MCFG in the range (0.03-8 [micro]g/mL).
albicans was achieved at 0.78 mg/mL, while the MIC of antifungal drugs of FLC and MCFG were 2 and 0.0156 (mg/mL) respectively.
Synergistic effects were obtained using various combinations of EOMS with MCFG and FLC with FICIs of 0.53 and 0.37, respectively.
albicans) (Figure 4(a)), a cell treated with EOMS (Figure 4(b)), FLC (Figure 4(c)), and MCFG (Figure 4(e)) alone, or a combination of EOMS plus FLC
The threat of increasing resistance to azole drugs, associated with the relative scarcity of antifungal drugs, prompted the development of new drugs such as the echinocandins (e.g., MCFG).
Secondly, we sought to evaluate the effect of combining these essential oil and synthetic drugs (FLC and MCFG) with a view to reduce the concentration of FLC and MCFG necessary to achieve a particular level of growth inhibition against Candida.
Our results indicate that using various combinations of EOMS with FLC and MCFG was observed synergistic effects.
Further demonstration of the existence of synergism between EOMS and conventional antifungal drugs (FLC and MCFG) was obtained by TEM observations (Figure 4), where the complete destruction of cytoplasmatic material of Candida cells (probably the induction of cell death) became evident in Figure 4(d).
Brisbane, Australia, Jan 31, 2011 - (ABN Newswire) - Texon Petroleum Limited (ASX:TXN) advises that its first Eagle Ford Well, Tyler Ranch EFS #1H, has produced 24,862 bo + 32,006 mcfg
over 45 days on a restricted choke.
Brisbane, Australia, Jan 17, 2011 - (ABN Newswire) - Texon Petroleum Limited (ASX:TXN) advises that its first Eagle Ford Well, Tyler Ranch EFS #1H, has produced 19,651 boe (17,814 bo + 22,052 mcfg
) over 30 days on a restricted choke.